MicroRNA-induced transdifferentiation
We’ve written intensively about direct transdifferentiation induced by defined factors. The most protocols in this field are using transcription factors as a tool. Recently, few groups have...
View ArticleGMP-compliant methods of human pluripotent stem cells cryopreservation
New open access journal BioResearch, basically is transforming into new “stem cell journal”. I’ve picked a recent review of cryopreservation methods for human pluripotent stem cell banks. This the...
View ArticleMolecular players in cellular reprogramming – a review
Molecualar Cell journal features a review by Thomas Vierbuchen and Marius Wernig – Molecular Roadblocks for Cellular Reprogramming. If you’re interested in iPS cells, reprogramming and...
View ArticleSynthetic feeder mimetics for pluripotent stem cell culture
Development of synthetic substrates is very important for clinical-grade feeder-free and xeno-free culture of pluripotent stem cells (PSC). We touched these issues last year. Today I’d like to share...
View ArticleClik here to view.
Risk of genotoxicity in human iPS cell-based therapeutics
We wrote a lot about risks associated with potential therapeutic application of iPS cells. Generation of iPS cells linked to significant genomic instability and acquisition of mutations. The recent...
View ArticleCurrent approaches to reprogram cell identity
The recent review by Samantha A Morris and George Daley about technologies for reprogramming of cell identity is very interesting and the most comprehensive. They nicely covered the history of cell...
View ArticleISCT’s position on potency assay development for cellular products
Potency assay is important and even critical part of cell product characterization. Potency determines quality of cellular product and underlie the mechanism of therapeutic action (MOA). International...
View ArticleOverview of cell separation technologies
The recent review Stem cell separation technologies, comprehensively covers all current cell separation methods. The review includes classification of cell separation, overview of methods, pros and...
View ArticleSynthetic surfaces for pluripotent stem cell culture
I’d like to share new and very interesting review, published in Cell Regeneration. The authors address the question of pluripotent stem cell interaction with surfaced used in cell culture via adhesion...
View ArticleAnalysis of MSC clinical preparation protocols
The recent review analyzes published mesenchymal stromal cells (MSC) preparations for clinical trials. 47 clinical protocols were analyzed, published from 2007 to 2013. I’d encourage you to read full...
View Article
